Bioprocessing:Article Title: Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pAAV-mDlx-NLS-mRuby2 addgene #99130; RRID:Addgene_99130 pAAV-flex-taCasp3-TEVp addgene #45580; RRID:Addgene_45580 pSADdG/CreGFP Charité Viral Vector Core BR-26 pN2cdG/CreGFP Charité Viral Vector Core BR-41a pAAV.Syn.NES-jRGECO1a.WPRE.SV40 addgene #100854; RRID:Addgene_100854 pAAV-CaMKIIahChR2(H134R)-mCherry (AAV2) UNC Vector Core N/A pAAV-CaMKIIa-mCherry (AAV2) addgene 114469- AAV2; RRID:Addgene_114469 pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (AAV 9) addgene 124651-AAV9; RRID:Addgene_124651 Biological samples Patient-derived xenografts (PDX) This paper N/A Chemicals, peptides, and recombinant proteins Poly-L-lysine Sigma P4707 Neurobasal Medium Gibco 11570556 Hibernate -A Medium Gibco A1247501 B-27 Supplement for neuronal co-culture Gibco 17504044 B27 supplement without vitamin A for GB culture Gibco 12587010 L-glutamine (GlutaMAX -I (100x)) Gibco 35050038 Antibiotic-Antimycotic (100x) Gibco 15240062 MgSO4 Sigma-Aldrich M3409 DMEM/F12 Medium Gibco 11330032 Insulin solution human Sigma I9278 Heparin Sigma H4784 EGF Biotechne 236-EG-200 FGF Recombinant Protein Life Technologies PHG0021 FGF Recombinant Protein 1mg Life Technologies PHG0023 Accutase Solution Thermo Fisher Scientific A1110501 0.05% Trypsin-EDTA (1x) Gibco 25300-054 2.5% Trypsin (10x) Gibco 15090-046 NGF Miltenyi Biotech 130-127-430 mTeSR Plus StemCell Technologies #100-0276 ReLeaSR StemCell Technologies #05872 1% N2 supplement Gibco 17502048 Minimum Essential Medium from non-essential amino acids Gibco 11140050 Laminin Thermo Fisher Scientific 23017015 BDNF Peprotech #450-02 NT-3 Peprotech #450-03 Doxycycline-HCl Thermo Fisher Scientific 15473189 Cytosine arabinoside Sigma #C6645 HyClone FBS Cytiva SH30071.03HI FBS Anprotec AC-SM-0041 AcX invitrogen A20770 Ammoniumpersulfate Sigma A3678 TEMED Merck T9281 4-Hydroxy-TEMPO Merck 176141 Acrylamide Sigma A9099 Sodiumacrylate Sigma 408220 Neurobiotin Tracer Vector Laboratories SP-1120 (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–22.e1–e20, January 23, 2025 Please cite this article in press as: Tetzlaff et al., Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing, Cell (2025), https://doi.org/10.1016/j.cell.2024.11.002 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER HEPES Sigma-Aldrich 7365-45-9 HEPES solution Sigma-Aldrich H0887 D-Glucose Sigma-Aldrich G8644 N-Methyl-D-Glucamin Sigma-Aldrich M2004 ASC acid Riedel-de-Haën 33034 HI horse serum ThermoFischer 26050-070 N,N’-Methylenbisacrylamide Merck 146072 Perampanel Eisai N/A Perampanel BioCrick BCC1847; CAS: 380917-97-5 CNQX MedChemExpress HY-15066A; CAS: 479347-85-8 NBQX HelloBio HB0443; CAS: 479347-86-9 TTX HelloBio HB1035; CAS: 18660-81-6 GI254023X Sigma-Aldrich SML0789 Gabazine Tocris CAS: 104104-50-9 Glutamate Sigma-Aldrich G8415 Acetylcholine Sigma-Aldrich A6625 5-HT Sigma-Aldrich 14927 GABA Sigma-Aldrich A2129 Adrenaline Tocris 5169 Dopamine hydrochloride Sigma-Aldrich H8502 Glycine Sigma-Aldrich G7126 ATP magnesium salt Sigma-Aldrich A9187 Alexa Fluor 594 hydrazide sodium salt invitrogen A10442 Atropine sodium salt Sigma-Aldrich A0132 Dulbecco’s Phosphate Buffered Saline Sigma-Aldrich D8537 Millicell cell culture inserts 0.4 um Merck PICM0RG50 CellTiter-Glo Luminescent Cell Viability Assay Promega G7570 NaCl Sigma-Aldrich S7653 KCL Fluka 60129 NaHCO3 Fluka 71627 NaH2PO4 Fluka 71496 Triton X-100 Sigma-Aldrich T9284 L-glutamine (GlutaMAX -I (100x)) Gibco 35050-038 Evans Blue Sigma E2129 SlowFade Gold Antifade Mountant ThermoFischer S36936 DAPI Sigma D9542 PFA 4% Roth P087.3 Sulforhodamine 101 (SR101) Sigma-Aldrich S7635 Tetramethylrhodamine isothiocyanate dextran (TRITC) Sigma-Aldrich 52194 Human WT Probes v2, RHS 10X Genomics 2000657 Human WT Probes v2, LHS 10X Genomics 2000658 Visium CytAssist Spatial Gene Expression for FFPE 10X Genomics 1000520 Dual Index Kit TS Set A, 96 rxns 10X Genomics PN-1000251 Poly-Prep Slides Sigma-Aldrich P0425-72EA Eosin Y-solution, Alcoholic Millipore HT110116 Hematoxylin Solution, Mayer’s Millipore MHS16 Bluing Reagent, Dako Agilent CS70230-2 KAPA SYBR FAST qPCR Master Mix KAPA Biosystems KK4600 Qubit 13 dsDNA HS Kit ThermoFischer Q33231 (Continued on next page) ll OPEN ACCESS Cell 188, 1–22.e1–e20, January 23, 2025 e3 Please cite this article in press as: Tetzlaff et al., Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing, Cell (2025), https://doi.org/10.1016/j.cell.2024.11.002 Article
Recombinant:Article Title: Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pAAV-mDlx-NLS-mRuby2 addgene #99130; RRID:Addgene_99130 pAAV-flex-taCasp3-TEVp addgene #45580; RRID:Addgene_45580 pSADdG/CreGFP Charité Viral Vector Core BR-26 pN2cdG/CreGFP Charité Viral Vector Core BR-41a pAAV.Syn.NES-jRGECO1a.WPRE.SV40 addgene #100854; RRID:Addgene_100854 pAAV-CaMKIIahChR2(H134R)-mCherry (AAV2) UNC Vector Core N/A pAAV-CaMKIIa-mCherry (AAV2) addgene 114469- AAV2; RRID:Addgene_114469 pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (AAV 9) addgene 124651-AAV9; RRID:Addgene_124651 Biological samples Patient-derived xenografts (PDX) This paper N/A Chemicals, peptides, and recombinant proteins Poly-L-lysine Sigma P4707 Neurobasal Medium Gibco 11570556 Hibernate -A Medium Gibco A1247501 B-27 Supplement for neuronal co-culture Gibco 17504044 B27 supplement without vitamin A for GB culture Gibco 12587010 L-glutamine (GlutaMAX -I (100x)) Gibco 35050038 Antibiotic-Antimycotic (100x) Gibco 15240062 MgSO4 Sigma-Aldrich M3409 DMEM/F12 Medium Gibco 11330032 Insulin solution human Sigma I9278 Heparin Sigma H4784 EGF Biotechne 236-EG-200 FGF Recombinant Protein Life Technologies PHG0021 FGF Recombinant Protein 1mg Life Technologies PHG0023 Accutase Solution Thermo Fisher Scientific A1110501 0.05% Trypsin-EDTA (1x) Gibco 25300-054 2.5% Trypsin (10x) Gibco 15090-046 NGF Miltenyi Biotech 130-127-430 mTeSR Plus StemCell Technologies #100-0276 ReLeaSR StemCell Technologies #05872 1% N2 supplement Gibco 17502048 Minimum Essential Medium from non-essential amino acids Gibco 11140050 Laminin Thermo Fisher Scientific 23017015 BDNF Peprotech #450-02 NT-3 Peprotech #450-03 Doxycycline-HCl Thermo Fisher Scientific 15473189 Cytosine arabinoside Sigma #C6645 HyClone FBS Cytiva SH30071.03HI FBS Anprotec AC-SM-0041 AcX invitrogen A20770 Ammoniumpersulfate Sigma A3678 TEMED Merck T9281 4-Hydroxy-TEMPO Merck 176141 Acrylamide Sigma A9099 Sodiumacrylate Sigma 408220 Neurobiotin Tracer Vector Laboratories SP-1120 (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–22.e1–e20, January 23, 2025 Please cite this article in press as: Tetzlaff et al., Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing, Cell (2025), https://doi.org/10.1016/j.cell.2024.11.002 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER HEPES Sigma-Aldrich 7365-45-9 HEPES solution Sigma-Aldrich H0887 D-Glucose Sigma-Aldrich G8644 N-Methyl-D-Glucamin Sigma-Aldrich M2004 ASC acid Riedel-de-Haën 33034 HI horse serum ThermoFischer 26050-070 N,N’-Methylenbisacrylamide Merck 146072 Perampanel Eisai N/A Perampanel BioCrick BCC1847; CAS: 380917-97-5 CNQX MedChemExpress HY-15066A; CAS: 479347-85-8 NBQX HelloBio HB0443; CAS: 479347-86-9 TTX HelloBio HB1035; CAS: 18660-81-6 GI254023X Sigma-Aldrich SML0789 Gabazine Tocris CAS: 104104-50-9 Glutamate Sigma-Aldrich G8415 Acetylcholine Sigma-Aldrich A6625 5-HT Sigma-Aldrich 14927 GABA Sigma-Aldrich A2129 Adrenaline Tocris 5169 Dopamine hydrochloride Sigma-Aldrich H8502 Glycine Sigma-Aldrich G7126 ATP magnesium salt Sigma-Aldrich A9187 Alexa Fluor 594 hydrazide sodium salt invitrogen A10442 Atropine sodium salt Sigma-Aldrich A0132 Dulbecco’s Phosphate Buffered Saline Sigma-Aldrich D8537 Millicell cell culture inserts 0.4 um Merck PICM0RG50 CellTiter-Glo Luminescent Cell Viability Assay Promega G7570 NaCl Sigma-Aldrich S7653 KCL Fluka 60129 NaHCO3 Fluka 71627 NaH2PO4 Fluka 71496 Triton X-100 Sigma-Aldrich T9284 L-glutamine (GlutaMAX -I (100x)) Gibco 35050-038 Evans Blue Sigma E2129 SlowFade Gold Antifade Mountant ThermoFischer S36936 DAPI Sigma D9542 PFA 4% Roth P087.3 Sulforhodamine 101 (SR101) Sigma-Aldrich S7635 Tetramethylrhodamine isothiocyanate dextran (TRITC) Sigma-Aldrich 52194 Human WT Probes v2, RHS 10X Genomics 2000657 Human WT Probes v2, LHS 10X Genomics 2000658 Visium CytAssist Spatial Gene Expression for FFPE 10X Genomics 1000520 Dual Index Kit TS Set A, 96 rxns 10X Genomics PN-1000251 Poly-Prep Slides Sigma-Aldrich P0425-72EA Eosin Y-solution, Alcoholic Millipore HT110116 Hematoxylin Solution, Mayer’s Millipore MHS16 Bluing Reagent, Dako Agilent CS70230-2 KAPA SYBR FAST qPCR Master Mix KAPA Biosystems KK4600 Qubit 13 dsDNA HS Kit ThermoFischer Q33231 (Continued on next page) ll OPEN ACCESS Cell 188, 1–22.e1–e20, January 23, 2025 e3 Please cite this article in press as: Tetzlaff et al., Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing, Cell (2025), https://doi.org/10.1016/j.cell.2024.11.002 Article
Retrograde Tracing:Article Title: Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER pAAV-mDlx-NLS-mRuby2 addgene #99130; RRID:Addgene_99130 pAAV-flex-taCasp3-TEVp addgene #45580; RRID:Addgene_45580 pSADdG/CreGFP Charité Viral Vector Core BR-26 pN2cdG/CreGFP Charité Viral Vector Core BR-41a pAAV.Syn.NES-jRGECO1a.WPRE.SV40 addgene #100854; RRID:Addgene_100854 pAAV-CaMKIIahChR2(H134R)-mCherry (AAV2) UNC Vector Core N/A pAAV-CaMKIIa-mCherry (AAV2) addgene 114469- AAV2; RRID:Addgene_114469 pAAV-CamKIIa-ChrimsonR-mScarlet-KV2.1 (AAV 9) addgene 124651-AAV9; RRID:Addgene_124651 Biological samples Patient-derived xenografts (PDX) This paper N/A Chemicals, peptides, and recombinant proteins Poly-L-lysine Sigma P4707 Neurobasal Medium Gibco 11570556 Hibernate -A Medium Gibco A1247501 B-27 Supplement for neuronal co-culture Gibco 17504044 B27 supplement without vitamin A for GB culture Gibco 12587010 L-glutamine (GlutaMAX -I (100x)) Gibco 35050038 Antibiotic-Antimycotic (100x) Gibco 15240062 MgSO4 Sigma-Aldrich M3409 DMEM/F12 Medium Gibco 11330032 Insulin solution human Sigma I9278 Heparin Sigma H4784 EGF Biotechne 236-EG-200 FGF Recombinant Protein Life Technologies PHG0021 FGF Recombinant Protein 1mg Life Technologies PHG0023 Accutase Solution Thermo Fisher Scientific A1110501 0.05% Trypsin-EDTA (1x) Gibco 25300-054 2.5% Trypsin (10x) Gibco 15090-046 NGF Miltenyi Biotech 130-127-430 mTeSR Plus StemCell Technologies #100-0276 ReLeaSR StemCell Technologies #05872 1% N2 supplement Gibco 17502048 Minimum Essential Medium from non-essential amino acids Gibco 11140050 Laminin Thermo Fisher Scientific 23017015 BDNF Peprotech #450-02 NT-3 Peprotech #450-03 Doxycycline-HCl Thermo Fisher Scientific 15473189 Cytosine arabinoside Sigma #C6645 HyClone FBS Cytiva SH30071.03HI FBS Anprotec AC-SM-0041 AcX invitrogen A20770 Ammoniumpersulfate Sigma A3678 TEMED Merck T9281 4-Hydroxy-TEMPO Merck 176141 Acrylamide Sigma A9099 Sodiumacrylate Sigma 408220 Neurobiotin Tracer Vector Laboratories SP-1120 (Continued on next page) ll OPEN ACCESS e2 Cell 188, 1–22.e1–e20, January 23, 2025 Please cite this article in press as: Tetzlaff et al., Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing, Cell (2025), https://doi.org/10.1016/j.cell.2024.11.002 Article .. REAGENT or RESOURCE SOURCE IDENTIFIER HEPES Sigma-Aldrich 7365-45-9 HEPES solution Sigma-Aldrich H0887 D-Glucose Sigma-Aldrich G8644 N-Methyl-D-Glucamin Sigma-Aldrich M2004 ASC acid Riedel-de-Haën 33034 HI horse serum ThermoFischer 26050-070 N,N’-Methylenbisacrylamide Merck 146072 Perampanel Eisai N/A Perampanel BioCrick BCC1847; CAS: 380917-97-5 CNQX MedChemExpress HY-15066A; CAS: 479347-85-8 NBQX HelloBio HB0443; CAS: 479347-86-9 TTX HelloBio HB1035; CAS: 18660-81-6 GI254023X Sigma-Aldrich SML0789 Gabazine Tocris CAS: 104104-50-9 Glutamate Sigma-Aldrich G8415 Acetylcholine Sigma-Aldrich A6625 5-HT Sigma-Aldrich 14927 GABA Sigma-Aldrich A2129 Adrenaline Tocris 5169 Dopamine hydrochloride Sigma-Aldrich H8502 Glycine Sigma-Aldrich G7126 ATP magnesium salt Sigma-Aldrich A9187 Alexa Fluor 594 hydrazide sodium salt invitrogen A10442 Atropine sodium salt Sigma-Aldrich A0132 Dulbecco’s Phosphate Buffered Saline Sigma-Aldrich D8537 Millicell cell culture inserts 0.4 um Merck PICM0RG50 CellTiter-Glo Luminescent Cell Viability Assay Promega G7570 NaCl Sigma-Aldrich S7653 KCL Fluka 60129 NaHCO3 Fluka 71627 NaH2PO4 Fluka 71496 Triton X-100 Sigma-Aldrich T9284 L-glutamine (GlutaMAX -I (100x)) Gibco 35050-038 Evans Blue Sigma E2129 SlowFade Gold Antifade Mountant ThermoFischer S36936 DAPI Sigma D9542 PFA 4% Roth P087.3 Sulforhodamine 101 (SR101) Sigma-Aldrich S7635 Tetramethylrhodamine isothiocyanate dextran (TRITC) Sigma-Aldrich 52194 Human WT Probes v2, RHS 10X Genomics 2000657 Human WT Probes v2, LHS 10X Genomics 2000658 Visium CytAssist Spatial Gene Expression for FFPE 10X Genomics 1000520 Dual Index Kit TS Set A, 96 rxns 10X Genomics PN-1000251 Poly-Prep Slides Sigma-Aldrich P0425-72EA Eosin Y-solution, Alcoholic Millipore HT110116 Hematoxylin Solution, Mayer’s Millipore MHS16 Bluing Reagent, Dako Agilent CS70230-2 KAPA SYBR FAST qPCR Master Mix KAPA Biosystems KK4600 Qubit 13 dsDNA HS Kit ThermoFischer Q33231 (Continued on next page) ll OPEN ACCESS Cell 188, 1–22.e1–e20, January 23, 2025 e3 Please cite this article in press as: Tetzlaff et al., Characterizing and targeting glioblastoma neuron-tumor networks with retrograde tracing, Cell (2025), https://doi.org/10.1016/j.cell.2024.11.002 Article
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